AQA GCSE Measuring & calculating rates of photosynthesis (Biology)

Measuring & calculating rates of photosynthesis

The rate of photosynthesis cannot be measured directly, so we measure something linked to it such as:

1.Counting oxygen bubbles released by pondweed per minute

2.Measuring the volume of oxygen produced using a gas syringe

Oxygen is produced during photosynthesis, so more oxygen = a faster rate.

Formula to calculate the rate of photosynthesis

Example

Counting bubbles:

If 24 bubbles are counted over 2 minutes

Rate = number of bubbles/time

Rate = 24/2 =12 bubbles per minute

Measuring volume of gas:

If 1.5cm3 of oxygen gas is collected over 10 minutes

Rate = volume of oxygen gas/time

Rate = 1.5/10 = 0.15 cm3/min

 

Rearranging this formula

Rate of photosynthesis formula rearranged
Rearrangement of rate of photosynthesis formula

Plotting Graphs Correctly

When drawing a graph, you must:

When drawing a graph, you must:

Axes

Choose a sensible scale that uses most of the graph

Use equal intervals

Label both axes with units

Plotting

Plot points accurately

Draw a smooth curve of best fit or straight line of best fit

Do NOT join dot to dot

Sample Data: Rate of Photosynthesis (Bubble Count Method)

Independent variable: Light intensity (arbitrary units / lamp distance)
Dependent variable: Rate of photosynthesis (bubbles per minute)

Rate of photosynthesis practical with pond weed
Light intensity (units)Bubbles per minute
00
103
207
3011
4014
5015
6015
Rate of photosynthesis graph vs light intensity

Example questions on these graphs

1.Describe the relationship between light intensity and rate of photosynthesis.

As light intensity increases rate of photosynthesis increases up to a maximum value. 

2.Identify the limiting factor between 0–30 units of light intensity.

Light intensity

3.Explain why the rate does not increase beyond 50 units.

Light intensity is no longer a limiting factor

4.What is the rate of photosynthesis at 40 units of light intensity?

14 bubbles per minute

Practice Questions

1.State two ways that we can measure the rate of photosynthesis

2. A graph is plotted using volume of oxygen gas produced against time. Suggest how the rate of photosynthesis can be measured and calculated at a specific point in time

3. In the diagram of the experiment above a tank of water is placed between the lamp and the pond weed. Suggest the role of the water tank.