Limiting factors interacting
Make sure you have read through the page on single limiting factors first, it can be found here.
A limiting factor is a factor that is in short supply, increasing the limiting factors will increase the rate of photosynthesis.
There are four limiting factors for photosynthesis
1.Temperature
2.Carbon dioxide concentration
3. Light intensity
4. Amount of chlorophyll
Photosynthesis depends on all factors at the same time. Increasing one factor only increases the rate if the others are not limiting.
Changing one factor can change which factor is limiting
Two-factor graphs
On the graph below each line shows how rate of photosynthesis changes with light intensity at a specific CO₂ concentration.
Differences between the lines show the effect of CO₂ concentration on the rate of photosynthesis.
Green part of the line
Light intensity is the limiting factor during the green part of the line. This is because as light intensity increases, so does the rate of photosynthesis.
Red part of the line.
Light intensity is no longer a limiting factor during either of the red parts of the graph line, because as light intensity increases, rate of photosynthesis is constant.
During the lower red line (0.01% carbon dioxide)
Carbon dioxide is the limiting factor, because as carbon dioxide increases, to 0.04% then rate of photosynthesis increases
During the higher red line (0.04% carbon dioxide)
Neither carbon dioxide concentration or light intensity are a limiting factor. Another factor, possible temperature is now limiting.
Three-factor graphs
In the graph below in the rising green line section:
In the green section of all the graph lines, light intensity is the limiting factor.
Increasing light provides more energy for the light-dependent reactions, increasing the rate of photosynthesis. Carbon dioxide concentration and temperature do not limit the rate at this stage, as the rate continues to increase when light intensity increases.
In the graph below at the plateau section (in red):
In all of the red lines, light intensity is no longer a limiting factor because as light intensity increases, rate of photosynthesis is constant.
Line 1: 0.01% CO₂, 15 °C to line 2 0.01% CO₂, 25 °C
The same carbon dioxide concentration is present, but temperature has increased, causing rate of photosynthesis to increase, so temperature is the limiting factor here during line 1.
Line 2: 0.01% CO₂, 25 °C to line 4 0.04% CO₂, 25 °C
Same temperature, but carbon dioxide concentration has increased, causing rate of photosynthesis to increase. So, carbon dioxide concentration will be a limiting factor during line 2.
Line 4:
High CO₂ concentration & optimum temperature
Rate of photosynthesis is highest possible rate on the graph.
In the red section of the highest line, light intensity, temperature and carbon dioxide are not limiting because all are at high levels.
The rate has reached a maximum for these conditions. It is not possible to conclude from the data that chlorophyll is the limiting factor, as the amount of chlorophyll was not varied. Additional data would be required.
Practice Questions
1.What is meant by limiting factor in photosynthesis?
2. Why does increasing light intensity not always increase the rate of photosynthesis?
3.If light intensity is high but carbon dioxide concentration is low, which factor limits photosynthesis?
4. Why must all factors be considered together when studying photosynthesis?
5.What does a plateau on a photosynthesis rate graph show?
6.A graph shows rate of photosynthesis against light intensity at low and high CO₂.
6a)At low light intensity, which factor is limiting for both curves?
6b)On the same graph, why does the curve at high CO₂ reach a higher maximum rate?
6c)Why do both curves eventually plateau even at high light intensity?
6d)A student increases CO₂ concentration but sees no increase in photosynthesis rate. Give a possible limiting factor.
7.How does low temperature limit the rate of photosynthesis?
8. Why does photosynthesis decrease at temperatures above the optimum?
Factors affecting the rate of photosynthesis
Measuring & calculating rates of photosynthesis
Inverse square law and photosynthesis
Economics of enhancing the conditions in greenhouses
Investigating the effect of light intensity on the rate of photosynthesis